Design of primers and probes is one of the most crucial factors affecting the success and quality of quantitative real-time PCR (qPCR) analyses, since an accurate and reliable quantification depends on using efficient primers and probes. Design of primers and probes should meet several criteria to f … Design of high-quality primers for multiple target sequences is essential for qPCR experiments, but is challenging due to the need to consider both homology tests on off-target sequences and the same stringent filtering constraints on the primers.
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Wei-Li Ling, Yuen-Ling Ng, Anil Wipat, David Philip Lane, Samuel Ken-En Gan, The quantification of antibody elements and receptors subunit expression using qPCR: The design of VH, VL, CH, CL, FcR subunits primers for a more holistic view of the immune system, Journal of Immunological Methods, Volume 476, 2020, 112683, ISSN 0022-1759, https ...
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Jun 11, 2019 · Validating a quantitative PCR (qPCR) experiment to minimize error and maximize data quality. A , nonvalidated primers could give variable and/or artefactual data that do not reflect the true target abundance in each sample.